Taub Institute: Genomics Core
AN NIA-FUNDED ALZHEIMER'S DISEASE RESEARCH CENTER
top
make an appointment

TaubCONNECT Research Perspectives:
June 2015





View Archive [close menu]

» #1 Making Cognitive Latent Variables Manifest: Distinct Neural Networks For Fluid
   Reasoning And Processing Speed

» #2 Functional Network Mediates Age-related Differences in Reaction Time: a Replication
   and Extension Study

» #3 Self-Efficacy Buffers the Relationship between Educational Disadvantage
   and Executive Functioning

» #1 Specific Downregulation of Hippocampal ATF4 Reveals a Necessary
   Role in Synaptic Plasticity and Memory

» #2 Mediterranean Diet and Leukocyte Telomere Length in a Multi-ethnic
   Elderly Population

» #1 Cerebellum can serve as a pseudo-reference region in Alzheimer's disease to detect
   neuroinflammation measured with PET radioligand binding to translocator protein (TSPO)

» #2 Assembly and Interrogation of Alzheimer's Disease Genetic Networks Reveal Novel
   Regulators of Progression

» Neurotherapeutics: Rethinking Alzheimer's Disease Therapies

» #1 Dysregulation of microRNA-219 promotes neurodegeneration through
     post-transcriptional regulation of tau

» #2 Olfactory deficits predict cognitive decline and Alzheimer dementia in an urban
     community

» #3 Increased permeability-glycoprotein inhibition at the human blood-brain
     barrier can be safely achieved by performing PET during peak plasma
     concentrations of tariquidar

» Regulation of synaptic plasticity and cognition by SUMO in normal physiology and
   Alzheimer's disease


» Lobar Microbleeds Are Associated with a Decline in Executive Functioning in
   Older Adults

» Targeting Axonal Protein Synthesis in Neuroregeneration and Degeneration

» Inbreeding among Caribbean Hispanics from the Dominican Republic and its effects on
   risk of Alzheimer disease

» Coding mutations in SORL1 and Alzheimer's disease

» First Place: Pathogenic Role of Formin-mediated Stable Detyrosinated
     Microtubule Inductionby Amyloid beta

» First Place: Differential responsiveness to entorhinal cortical input distinguishes
    CA1 pyramidal neuron subpopulations

» Soluble amyloid beta levels are elevated in the white matter of Alzheimer's patients,
   independent of cortical plaque severity

» A Time Course Analysis of the Electrophysiological Properties of Neurons Differentiated
   from Human Induced Pluripotent Stem Cells (iPSCs)

» Axonally Synthesized ATF4 Transmits a Neurodegenerative Signal across Brain Regions

» Olfactory Dysfunction in the Elderly: Basic Circuitry and Alterations with Normal Aging
   and Alzheimer's Disease

» Neurological disorders: Quality-control pathway unlocked

» Estrogen Receptor Œ± Variants Modify Risk for Alzheimer's Disease in a Multiethnic
   Female Cohort

» Combined suppression of CASP2 and CASP6 protects retinal ganglion cells from
   apoptosis and promotes axon regeneration through CNTF-mediated JAK/STAT
   signalling and Guidelines

» Local synthesis of TC10 is required for membrane expansion during axon outgrowth

» Biobanked Alzheimer's Brain Tissue Yields Living Neurons

» Behavioral Assays with Mouse Models of Alzheimer's Disease: Practical Considerations
   and Guidelines

» Biobanked Alzheimer's Brain Tissue Yields Living Neurons

» Picomolar Amyloid-Œ≤ Peptides Enhance Spontaneous Astrocyte Calcium Transients



SUMO1 Affects Synaptic Function, Spine Density and Memory


Ottavio Arancio, MD, PhD

Post-translational modifications constitute an important step in protein biosynthesis that regulates protein function by attaching functional groups to the amino-acids that compose the protein. This recent Scientific Reports article from the laboratory of Taub faculty member Dr. Ottavio Arancio, in collaboration with the Fraser Laboratory at the Tanz Centre for Research in Neurodegenerative Diseases of the University of Toronto, focuses on protein post-translational modification by Small Ubiquitin-like Modifier-1 (SUMO1). Matsuzaki et al. report the development of a unique transgenic mouse model which has permitted the identification of novel contributions of SUMO1 to synaptic transmission and the stability of dendritic spines. These changes in neuronal form and function also translate into a previously unrecognized significant impairment in learning and memory. To support the mechanism of action underlying these changes, Matsuzaki et al. have identified a number of previously unrecognized SUMO1 conjugation candidates that have an impact on a variety of cellular pathways. These SUMO1 targets are linked to presynaptic function such as a protein called synaptotagmin, which leads to diminished neurotransmitter release. Another target, the cytoplasmic FMR1-interacting protein 1 (Cyfip1), regulates actin organization at synapses and is linked to autism as well as schizophrenia, suggesting a potentially broader impact of SUMO1 modifications on disease and development. The increase in SUMO1 also causes a substantial reduction in neuronal spine density and strength of cell to cell communication. The changes in neuronal function and morphology were also associated with a specific impairment in learning and memory while other behavioral features remained unchanged. These findings point to a significant contribution of SUMO1 modification on neuronal function, which may have implications for mechanisms involved in intellectual disability and neurodegeneration. As such, these results will have a large impact on the neuroscience field.

Previously, the Arancio Laboratory discovered that SUMO2 conjugation, the other type of SUMO conjugation, is reduced in brain tissue from Alzheimer's Disease (AD) patients. While neuronal activation normally induced up-regulation of SUMO2 conjugation, this effect was impaired by amyloid-beta, a toxic protein that is known to play a major role in AD. Amyloid-beta is also a known potent disruptor of synaptic function. Thus, taken together, these studies reveal a unique mechanism for the two types of SUMO, in which SUMO1 and 2 play opposite roles in cell to cell communication and memory. SUMO1 shows a negative impact on synaptic development/stability/plasticity/memory while SUMO2/3 is beneficial to these features.

FIGURE LEGEND: The SUMOylation Pathway. The SUMO enzymatic cascade catalyzes the dynamic post-translational modification process of transfer of SUMO protein to other proteins (SUMOylation). The Small Ubiquitin-related Modifier, SUMO is conjugated to its substrates through three sequential enzymatic steps: activation, involving the E1 enzyme, AOS1/UBA2; conjugation, involving the E2 enzyme, UBC9; substrate modification, through the cooperation of the protein ligases, E2 and E3. De-conjugation (De-SUMOylation) occurs through enzymes called SENPs, which recycle free SUMO and unmodified target proteins. The Arancio lab has demonstrated that SUMO1 conjugation negatively affects synaptic morphology, neurotransmission and memory, whereas SUMO2 conjugation positively affects these features. This work gives the impetus to the development of drugs acting at different steps of the pathway to alleviate Alzheimer's disease symptomatology.

Ottavio Arancio, MD, PhD
Oa1@columbia.edu



Connectivity and Circuitry in a Dish Versus in a Brain


Ottavio Arancio, MD, PhD

The complexity of the human central nervous system and its inaccessibility to direct studies make its modeling necessary in order to investigate the occurrence of physiological and pathological processes. Because of many limitations in animal disease models, in vitro neuronal models using patient-derived stem cells are currently being developed by numerous laboratories. However, this technological development has led to the need to confirm functionality of the newly generated neurons, with respect to electrical properties of individual neurons, their ability to express pathology, and their capacity to functionally integrate into the brain's circuitry. Additional problems of stem cell derived models include the survival of implanted neurons, the directed differentiation into specific cell types and the tumorigenic potential of incompletely differentiated neurons.

Figure 1: Immunofluorescence analyses of iPSC-derived neurons. Images by Andrew Sproul of the Taub Institute.

The laboratory of Taub faculty member Dr. Ottavio Arancio is at the forefront of research aimed at developing suitable neurons obtained with stem cell techniques. This is particularly true for cells mimicking diseases in which neurons have to be fully mature, such as those in patients with Alzheimer's disease. Dr. Arancio and colleagues recently published a review of studies from numerous laboratories, including the Arancio Laboratory, in the journal of Alzheimer's Research & Therapy. Their conclusion was that recapitulation of human neurological disease following stem cell-derived neuronal differentiation in vitro remains a significant scientific challenge. Nevertheless, the potential of discovering a therapy for numerous diseases awaiting a cure make it one worth pursuing, particularly for neurodegenerative diseases. To this end, maturation protocols that provide stem cell derived neuronal models containing genetic susceptibility in parallel with aging-related factors must continue to be developed in order to accurately model late-onset disorders.

Ottavio Arancio, MD, PhD
Oa1@columbia.edu










bottom bar