Human Immune Monitoring Core

The Human Immune Monitoring Core (HIMC) provides top-quality, cost-effective services for deep, comprehensive, multi-omics immune profiling and monitoring at single-cell resolution, enabled by cutting-edge technologies and our highly experienced and knowledgeable physicians, scientists, and technical team.

HIMC is innovation driven and project oriented. We bring the latest technologies for advanced immunology studies and develop the most robust assays and optimized methods on these platforms. Accommodating specific needs of each study and project, our expert team of physicians, scientists, and technicians will provide customizable, integrated services from experimental design, sample processing, data acquisition and processing, to data analysis and management. We apply stringent QC procedures and SOPs to deliver high-quality, accurate, and reproducible results within reasonable time and budget.

To get started, please contact Dr. Victor Guo, manager of HIMC at xg2295@columbia.edu or 212-305-6860.

For oversight, feedback, suggestions, please feel free to contact Dr. Victor Guo, or Dr. Charles Drake, Faculty Director of HIMC as you see appropriate. We appreciate your support and advice.

Our Services

Tissue: FFPE (including TMA), frozen tissue sections, isolated cells

Vectra

Quantitative multiplex immunofluorescent tissue imaging and spatial analysis using automated multispectral microscopy and advanced machine learning [more info]

NanoString

Highly sensitive, rapid, and precise multiplex gene profiling; FFPE or frozen tissue compatible [more info]

Single cell suspension: blood, digested/dissociated tissues, cryopreserved or fixed cells

CyTOF

High-parametric mass cytometry; up to 45+ markers and 40 barcoded samples in same tube; no compensation needed [more info]

Aurora

High-parametric spectral flow cytometry; up to ~30 colors in same tube [more info]

10x Genomics

Single-cell gene expression profiling, paired VDJ sequencing, and surface protein expression on same cells at scale [more info]
 
Single cell in situ:

iSpot

Advanced ELISpot and FluoroSpot reader; up to 3 colors [more info]

 

Technologies

HIMC offers the following technology platforms. To get started, please use the Service Request form. The FAQ & Resources page has additional information and answers to common questions, such as guidelines and requirements for sample preparation, billing, shipping, etc.

 

Vectra (quantitative multiplex immunofluorescent tissue imaging and spatial analysis):

  • up to 7 markers (including DAPI) using regular FFPE or TMA sections
  • automated multispectral imaging and component (marker) quantification
  • tissue and cell segmentation and phenotyping using advanced machine learning software
  • additional computational tools for data analysis

NanoString (highly sensitive, rapid, and precise multiplex gene profiling):

  • up to 800 genes, no amplification required
  • low sample input, FFPE compatible
  • rapid data output, no bioinformaticist required

CyTOF (high-parametric mass cytometry):

  • currently up to ~45 biological markers, plus barcoding capacity of up to 40 samples, in one staining and acquisition tube
  • minimal spillover between channels; compensation is not necessary
  • low background for intracellular and phospho staining
  • deep, comprehensive immune profiling and data analysis using advanced software and computational tools (SPADE, viSNE, PhenoGraph, etc)

Aurora (high-parametric spectral flow cytometry):

  • currently up to ~27 biological markers, upgradable to ~30-40 markers by early 2019
  • comprehensive immune profiling and data analysis using advanced software and computational tools (SPADE, viSNE, PhenoGraph, etc)

10x Genomics (single-cell gene expression profiling and paired VDJ sequencing at scale):

  • comprehensive, scalable gene expression profiling and characterization of thousands to millions of cells at single-cell resolution
  • paired TCR alpha and beta (and paired Ig heavy and light chains) sequencing
  • simultaneous scTCRseq, scRNAseq, and surface protein marker characterization (CITE-seq) on same single cells
  • no need for plate sorting

iSpot (multicolor ELISpot and FluoroSpot reader)

  • high quality, high speed, user friendly, currently up to 3 colors in same assay