Faculty and Staff
Nutritional and Metabolic Doctoral Training Faculty
Lloyd A. Greene, Ph.D.
Professor of Pathology
B.S. 1965, University of Chicago, Chicago
Ph.D. 1970, University of California, San Diego, La Jolla
The overall goal of research in this laboratory is to understand the mechanisms whereby neuronal precursors differentiate into mature functional neurons. To this end, we use the rat PC12 pheochromocytoma cell line developed in this laboratory as a model system to study the mechanism of action of nerve growth factor (NGF) and the steps that lead to neuronal differentiation. Among current projects in the laboratory are those addressing the following questions:
1) What is the essential property of the high-affinity NGF receptor that permits it to mediate the functional activities of NGF and how is this receptor different from the low-affinity, non-functional NGF receptor?
2) What is the transductional mechanism by which NGF receptor occupancy leads to subsequent response?
3) What are the steps beyond immediate transduction that lead to NGF mechanisms?
4) What is the mechanism by which NGF regulates growth cone motility? What are the molecules involved in this effect?
5) What genes are regulated by NGF? By what pathways are they regulated?
6) What are the mechanism by which NGF promotes the initiation and regeneration of neurites; specifically, what is the role of specific cytoskeleton proteins in this process?
7) How do NGF and other neuronotrophic substances maintain cell survival? 8) How do such agents regulate cell proliferation?
Recent Publications - Pubmed
Sproul AA, Xu Z, Wilhelm M, Gire S, Greene LA. Cbl negatively regulates JNK activation and cell death. Cell Res. 2009 Aug;19(8):950-61.
Li G, Li W, Angelastro JM, Greene LA, Liu DX. Identification of a novel DNA binding site and a transcriptional target for activating transcription factor 5 in c6 glioma and mcf-7 breast cancer cells. Mol Cancer Res. 2009 Jun;7(6):933-43. Epub 2009 Jun 16.
Zareen N, Greene LA. Protocol for culturing sympathetic neurons from rat superior cervical ganglia (SCG). J Vis Exp. 2009 Jan 30;(23). pii: 988. doi: 10.3791/988.
Lee HY, Greene LA, Mason CA, Manzini MC. Isolation and culture of post-natal mouse cerebellar granule neuron progenitor cells and neurons. J Vis Exp. 2009 Jan 16;(23). pii: 990. doi: 10.3791/990.