Gel Bands or spots for MALDI or LC-MS/MS analysis:

1.  Stain the gel using one of the recommended stains on our web site at http://cpmcnet.columbia.edu/dept/protein/ or another MS-compatible stain. Best results are obtained with Coomassie-stained gels.

2.  While wearing gloves, cut the gel spot or band with a clean blade and put it in an Eppendorf tube. Be sure the cap is closed securely. No additional liquid is necessary. The samples may be stored at –20 until ready to send.

Samples in solution for mass determination: Send a 10-20µl aliquot in an Eppendorf or screw-cap tube. Make sure it is sealed securely. Tubes may be sent in Dry Ice or, if the protein is stable, at room temperature.

Samples for Edman (N-terminal) sequencing: For PVDF bands, cut with a clean blade and place in a tube. Send them at room temperature overnight. For samples in solution, follow the instructions above for mass determination samples.

FOR ALL SAMPLES:

1. Label the tubes clearly with your name and sample name/number, and send them in a well-padded envelope by overnight express at room temperature to:
   
   Mary Ann Gawinowicz
   Protein Core Facility
   Columbia University
   160 Fort Washington Avenue, Room 415
   New York, NY 10032
   
   
2. Include a PO made out to:
   
   Protein Core Facility
   Box 54
   630 W. 168th Street
   New York, NY 10032
   
   
3. We need a hard copy of the PO before we will start the work;    this may be included with the sample or faxed to 212-305-1599.
4. Also include the correct billing address and the name of a contact person with phone# for PO and invoice inquiries.
5. Include approximate MW and species (MS samples only).
6. Include your name, phone number, fax number, and a shipping address for UPS delivery, room number included. 

All billing inquiries should be addressed to Phyllis Smith, pgs2013@columbia.edu, phone 212-305-3793.

| TOP |