|
The Columbia University Protein Core Facility provides a range of services to all Columbia researchers and to outside users, including mass spectrometry, protein sequencing, and DNA sequencing.
Note to our users: The Protein Core Facility has moved to 160 Fort Washington Avenue, Room 415. Samples may be brought directly to the lab or left in drop boxes at the following locations: P&S 16th floor, HHSC 7th floor, and PI Annex 6th floor. Samples will be collected daily.
Questions should be directed to Mary Ann Gawinowicz at 212-305-3631,
email: mag4@columbia.edu
Mass Spectrometry:Services available include protein identification from gel bands or spots by MALDI-TOF and ESI-LC-MS/MS, post-translational modification identification, and mass analysis of intact proteins. Mass spectrometry is used to identify interacting partners in protein complexes that have been purified by immunoprecipitation and other methods.The Core also routinely provides structural confirmation of expressed proteins and protein fragments for structural biology studies.
Protein Sequencing: N-terminal sequencing of proteins in solution or blotted to PVDF membranes is available.
DNA Sequencing: The core offers high quality, fast turnaround DNA sequencing of various types DNA - plasmids, PCR products, GC-rich templates, cosmids, and BACs. DNA sequencing results are distributed through a secure network server with automated email notification.
Ion Torrent Next Generation Sequencing (NGS) is now available at our core facility.
The Ion Torrent NGS, powered by semiconductor chip technology, delivers the fastest run times of any next-generation sequencing platform available today.
|
