The Columbia University Protein Core Facility provides a range of services to all Columbia researchers and to outside users, including mass spectrometry, protein sequencing, and DNA sequencing.

Note to our users:   The Protein Core Facility has moved to 160 Fort Washington Avenue, Room 415. Samples may be brought directly to the lab or left in drop boxes at the following locations:  P&S 16th floor, HHSC 7th floor, and  PI Annex 6th floor. Samples will be collected daily.

Questions: Call 212-305-3631 or e-mail mag4@columbia.edu  

Mass Spectrometry: Services available include protein identification from gel bands or spots by MALDI-TOF and ESI-LC-MS/MS, post-translational modification identification, and mass analysis of intact proteins. Mass spectrometry is used to identify interacting partners in protein complexes that have been purified by immunoprecipitation and other methods.The Core also routinely provides structural confirmation of expressed proteins and protein fragments for structural biology studies.                                         
Protein Sequencing: N-terminal sequencing of proteins in solution or blotted to PVDF membranes is available.

DNA Sequencing: The core offers high quality, fast turnaround DNA sequencing of various types DNA - plasmids, PCR products, GC-rich templates, cosmids, and BACs. DNA sequencing results are distributed through a secure network server with automated email notification.

To our users:

If you publish articles or receive grants using data generated in the Protein Core Facility, please acknowledge the Core's contributions for standard and non-standard services. Let us know when something is published or a grant is awarded so that we can track publications and grants. This is crucial for continued support both from external funding agencies and from internal institutional sources.