Selected Laboratory Protocols

Mouse/Atherosclerosis Protocols

• Genotyping of Asm-/- Mice
• Protocol for Bone Marrow Transplantation
• Principles of Mouse Husbandry:  George Kuriakose's March 2006 PowerPoint Presentation
• Assessment of Progression of Atherosclerotic Lesions in Mice
• Mouse Shipping Information
• Genotyping of ACAT-/- Mice
• Genotyping of F4/80-Cre Knock-In Mice
• Basic Information for Directing a Mouse Atherosclerosis Project
• Important Information About the Harlan-Teklad 88137 Western Diet Used in the Tabas Laboratory for Mouse Atherosclerosis Studies

Lipoprotein Protocols

• Protocol for Reconstitution of the Hydrophobic Core of Acetyl-LDL
• Labeling of Acetyl-LDL with DiI (1,1'-dioetadeeyl-3,3,3',3'-tetramethylindocarboeyanine perchlorate)
• Protocol for Preparation of LDL from Human Plasma
• Precautions to avoid endotoxin contamination of LDL and Acetyl-LDL

Cell Protocols

• Transfection of J774 Macrophages Using Fugene^® HD from Roche
• Preparation of Adherent Macrophages for Electron Microscopy
• Technical Points About Plasmid-Mediated Transfection or RNAi-Mediated Gene Knockdown in Methyl-BSA-Elicited Macrophages Using the Amaxa Nucleofector® System (Dr. Yankun Li, September, 2005)
• Evaporation from Cell Culture Plates (Nunc Bulletin)
• Concanavalin A-Elicited Mouse Peritoneal Macrophages
• Preparation of Peritoneal Macrophages from Methylated BSA-Immunized Mice
• Ted Mazzone's protocol for stable transfection of J774 cells by calcium phosphate co-precipitation
• Stability and Dose Curve of ACAT Inhibitor 58035 in ConA-Elicited Mouse Peritoneal Macrophages
• Filipin Fluorescence Staining of Free Cholesterol in Cultured Cells

Assays

• Immunoprecipitations Using TrueBlot
• GC Assay for Cellular Cholesterol Mass
• CHOP Immunoblots of Whole Cell Extracts
• Preparation of ¹⁴ C-Oleate-Albumin Solution for Whole-Cell Cholesterol Esterification Assay
• Method for Preparing Cytoplasmic and Nuclear Extracts for Immunoblots of UPR Proteins
• Assay for Apoptosis of FC-Loaded Macrophages (Annexin/propidium iodide kit is from Molecular Probes)  
• Extraction of Lipids in Solution by the Method of Bligh & Dyer

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last updated: June 14, 2005