My laboratory is investigating the biology of the human LINE-1 retrotransposable element. These DNA elements are members of a family of DNA elements that have been present in eukaryotic genomes for over 600 million years. They are capable of making new copies of themselves and inserting into new genomic loci. By amplifying themselves in this way, LINE-1 elements have come to make up about 15% of human DNA.
We have developed a new assay that is designed to compare the genomic DNA from different individuals or from different tissues from the same individuals and to identify loci that have LINE-1 insertions in some but not all of the samples. We are using this technique to: 1. study the structure of the human genome, 2. answer questions about human history and evolution, and 3. to investigate the role of human LINE-1 elements in the development of cancer.
Division of Molecular Medicine
|1976||B.A., Cornell University, Ithaca, NY .||1983||M.D., Ph.D., Albert Einstein College of Medicine, Bronx, NY|
|1998-present||Assistant Professor, Department of Medicine, Division of Molecular Medicine,
Columbia University, New York City,|
|1997-1998||Medical Director, Program in Cancer Genetics, Center for Medical Genetics|
|1993- 1998||Senior Staff Fellow, - FDA, Center for Biologics Evaluation and Research,
Division of Cellular and Gene Therapies|
|1990-1992||Fellow in Clinical Medical Genetics - National Institutes of Health|
|1990-1992||Senior Staff Fellow - National Cancer Institute, NIH, Laboratory of Dr.
|1987-1990||Medical Staff Fellow - National Cancer Institute, NIH, Lab of Dr. Maxine Singer|
|1986-1987||PRAT Fellow - National Institute of Diabetes and Digestive Diseases, NIH|
|1983-1986||Intern and Resident in Internal Medicine - Albert Einstein Medical
Center, Bronx, NY|
|1996-1998||Member, National Action Plan On Breast Cancer, Hereditary Susceptibility Working Group|
|1999||Project Award, Office of Clinical Trials, Columbia-Presbyterian Medical Center|
|1999||Pilot Project Award, Herbert Irving Comprehensive Cancer Center, Columbia-Presbyterian Medical Center|
|1998||Visiting Professor, Scranton-Temple Residency Program|
|1997||NIH Directors Award|
|1994-1996||Directors Targeted Research Award, FDA, Center for Biologics Evaluation and Research|
|1995||ASBMB High School Science Teacher Fellowship Sponsor|
|1990||U.S. Patent Number 4,901,381. Issued Feb. 20|
|1986-1988||Pharmacology Research Associate Fellow, NIH|
|1976-1983||NIH Medical Scientist Training Program Fellow|
|1976||B.A. with Distinction in All Subjects, Cornell University|
|1975||Alvan T. - Viola D. Fuller American Cancer Society Junior Research Fellowship|
|1973-1977||N.Y.S. Cornell Regents Scholarship Award|
|1972||Winner, National Westinghouse Science Talent Search|
|American College of Medical Genetics|
|American Society of Human Genetics|
|American Association for the Advancement of Science|
|American Society for Biochemistry and Molecular Biology (Elected Member, 1993)|
|American Society for Microbiology|
|Human Genome Organization|
Swergold, G.D. Identification, Characterization, and Cell Specificity of a Human LINE-1 Promoter. Mol. Cell. Biol., 10: 6718-6729. 1990.
Goldfarb, L.G., Brown, P., McCombie, W.R., Goldgaber, D., Swergold, G.D., Wills, P.R., Cerenakova, L., Baron, H., Gibbs Jr., C.J., and D.C. Gajdusek. Transmissible Familial Creutzfeldt-Jakob Disease Associated With Five, Seven, and Eight Extra Octapeptide Coding Repeats in the PRNP Gene. Proc. Nat. Acad. Sci., USA, 88:10926-10930. 1991.
Holmes, S., M.F. Singer, and G.D. Swergold. Studies on p40, the Leucine Zipper containing Protein Encoded by the First Open Reading Frame of an Active Human Transposable Element.. J. Biol. Chem.. 267: 19765-19768. 1992.
Goldfarb, Lev, et. al.. Fatal Familial Insomnia and Familial Creutzfeldt -Jakob disease: disease phenotype determined by a DNA polymorphism. Science 258: 806-808. 1992.
Becker, K., G.D. Swergold, K. Ozato, and R.E. Thayer. Binding of the ubiquitous nuclear transcription factor YY1 to a cis regulatory sequence in the human LINE-1 transposable element. Human Molecular Genetics. 2: 1697-1702. 1993
Singer, M.F., V. Krek, J.P. McMillan, G.D. Swergold, and R.E. Thayer. (1993) LINE-1: a human transposable element. Gene 135: 183-188. 1993
Rothenberg, K., B. Fuller, M. Rothstein, T. Duster, M.J. Ellis Kahn, R. Cunningham, K. Hudson, M.-C. King, P. Murphy, G. Swergold, and F. Collins. Genetic Information and the Workplace: Legislative Approaches and Policy Challenges. Science 275:1755-1757, 1997.
Sassaman, D.M., B.A. Dombroski, J.V. Moran, M.L. Kimberland, T.P. Naas, R.J. DeBerardinis, G.D. Swergold, and H.H. Kazazian Jr., Characterization of five novel active L1 elements allows an estimate of the number of active L1 elements in the human genome. Nature Genetics 16:37-43, 1997.
Deininger, P. L., S.T. Sherry, G. Risch, C. Donaldson, M.B. Robichaux, H. Soodyall, T. Jenkins, F. Sheen, G. Swergold, M. Stoneking and M.A. Batzer (In Press) Interspersed repeat insertion polymorphisms for studies of human molecular anthropology. In "Genomic Diversity: Applications in Human Population Genetics", S. S. Papiha, R. Deka and R. Chakraborty (Eds.), Plenum Press, New York, NY.
Fuller, B. P., M. J. Ellis Kahn, P.A. Barr, L. Biesecker, E. Crowley, J. Garber, M. K. Mansoura, P. Murphy, J. Phillips, K. Rothenberg, M. Rothstein, J. Stopfer, G. Swergold, B. Weber, F. S. Collins, and K. L. Hudson. Privacy in Genetics Research. Science, 285:1359-1361, 1999.