F A C U L T Y   P R O F I L E 

Steinberg

YAZAWA, MASAYUKI, PH.D.
Assistant Professor of Rehabilitation & Regenerative Medicine and Pharmacology

Tool development for live cell imaging & drug development for cardiac arrhythmia and cardiomyopathy.

Office: Physicians & Surgeons | 7th Floor | Room 431/421
Telephone: 212.305.1052/1890
Email:
my2387@cumc.columbia.edu

Current Research

Our research focuses on studying intracelleular calcium signaling in excitable cells such as myocytes and neurons and on developing new tools for cell biology. Our recent research has shown that human induced pluripotent stem cells derived from skin cells of patients with Timothy syndrome (due to gain-of-function mutations in calcium channel CACNA1C gene) can be differentiated into cardiomyocytes, allowing for detailed study of the cellular, biochemical and electrophysiological mechanisms underlying long QT syndrome and ventricular tachycardia that are found in these patients and providing a robust model for the testing of antiarrhythmic drugs. We have also developed light-activated systems called optogenetics for regulating protein-protein interactions that will aid in cell signaling research. Using human stem cell technology and imaging tools as well as animal models our laboratory will investigate how mutants in calcium-related molecules cause developmental disorders in human heart and brain in order to find new therapeutics.


Selected Publications

1. Quejada JR, Park SE, Awari DW, Shi F, Yamamoto H, Kawano F, Jung JC and Yazawa M. Optimized Light-inducible Transcription in Mammalian Cells using Flavin Kelch-repeat Fbox1/GIGANTEA and CRY2/CIB1. Nucleic Acids Research, in press.

2. Song LJ, Park SE, Isseroff Y, Morikawa K and Yazawa M. Inhibition of CDK5 alleviates the cardiac phenotypes in Timothy syndrome. Stem Cell Reports, July 11; 9(1)50-57. (2017). Epub 2017 June 22. PMID: 28648896.

3. Kawano F. Shi F and Yazawa M Optogenetics: Switching with red and blue. Nat. Chem. Biol. May 17;16(6):573-574. (2017). PMID: 28514429

4. Kawano F. Okazaki R, Yazawa M and Sato M. A photoactivatable Cre/loxP recombination system for optogenetic genome engineering. Nat. Chem. Biol. Dec;12(12):1059-1064 Epub 2016 Oct. 10. (2016). PMID: 27723747.

5. Song L, Awari DW, Han EY, Park SE, Uche-Anya E, Yabe YA, Chung WK, Yazawa M. Dual Optical Recordings for Action Potentials and Calcium Handling in Induced Pluripotent Stem Cell Model of Cardiac Arrhythmias Using Genetically Encoded Fluorescent Indicators. Stem Cells Translational Medicine. May;4(5):468-75. doi: 10.5966/sctm.2014-0245. Epub 2015 Mar 13. (2015). PMID: 25769651

6. Sun, N.*, Yazawa, M.*, Liu J, Han L, Sanchez-Freire V, Abilez OJ, Navarrete EG, Hu S, Wang L, Lee A, Pavlovic A, Lin S, Chen R, Hajjar RJ, Snyder MP, Dolmetsch RE, Butte MJ, Ashley EA, Longaker MT, Robbins RC, Wu JC. Patient-Specific Induced Pluripotent Stem Cell as A Model for Familial Dilated Cardiomyopathy. Science Translational Medicine. 4 (130), 130ra47 (2012). PMID: 22517884 * These authors contributed equally to this work.

7. Yazawa, M., Hsueh, B., Jia, X, Pasca, A.M., Bernstein, J.A., Hallmayer, J. and Dolmetsch, R.E. Using induced pluripotent stem cells to investigate cardiac phenotypes in Timothy syndrome. Nature 471, 230-234 (2011). PMID: 21307850

8. Yazawa, M., Sadaghiani, A.M., Hsueh, B. & Dolmetsch, R.E. Induction of protein-protein interactions in live cells using light. Nature Biotechnology. 27, 941-945 (2009). PMID: 19801976

9. Yazawa, M., Ferrante, C., Feng, J., Mio, K., Ogura, T., Zhang, M., Lin, PH., Pan, Z., Komazaki, S., Kato, K., Nishi, M., Zhao,X., Weisleder, N., Sato, C., Ma, J. and Takeshima, H. TRIC channels are essential for Ca2+ handling in intracellular stores. Nature 448, 78-82 (2007). PMID: 17611541