"Stealth
Cell" Time-lapse of GFP-integrin Chimera displayed in Rainbow style (earliest
image orange, followed by yellow, green, then purple), with post
fixation phaloidin stain (red).
"Stealth
Cell" Time-lapse of GFP-integrin Chimera displayed in Rainbow style (earliest
image orange, followed by yellow, green, then purple), with post
fixation phaloidin stain (red).
Rainbow style time-lapse
of a migrating cell.
Cell injected with antibody against glu microtubules (green)
that causes intermediate filament (red) colapse.
Serum starved fibroblasts orient their MTOC (visualized by pericentrin
staining in red) when treated with calf serum (CS) or lysophosphatidic
acid (LPA) but not when treated with serum free medium (SFM). Microtubules
are in green, DNA in blue.
a. Capped
microtubules do not grow as tyr microtubules (blue) incorporate exogenous
rhodamine tubulin (red) at their plus ends (arrows), but stable glu microtubules (green)
do not. b. Calcium treated glu microtubules (green) fragment and can incorporate
rhodamine tubulin (red) from the newly formed plus ends (arrows).
Cells
expressing DAD, a fragment of mDia2 which activates endogenous mDia, generate
stable glu microtubules (green) that are unable to incorporate microinjected
rhodamine tubulin (red). Note that rhodamine tubulin still incorporates
onto the end of tyr microtubules (blue).