A Single Cell Reference Map for Human Blood and Tissue T cell Activation

Latest CCTI Paper, in Nature Communications

In the abstract for their most recent paper, published in the October 17th issue of Nature Communications, the Farber lab submits that human T cells coordinate adaptive immunity in diverse anatomic compartments through production of cytokines and effector molecules, but it is unclear how tissue site influences T cell persistence and function. They used single cell RNA-sequencing (scRNA-seq) to define the heterogeneity of human T cells isolated from lungs, lymph nodes, bone marrow and blood, and their functional responses following stimulation. Through analysis of >50,000 resting and activated T cells, they reveal tissue T cell signatures in mucosal and lymphoid sites, and lineage-specific activation states across all sites including distinct effector states for CD8+ T cells and an interferon-response state for CD4+ T cells. Comparing scRNA-seq profiles of tumor-associated T cells to their dataset revealed predominant activated CD8+compared to CD4+ T cell states within multiple tumor types. Their results therefore establish a high dimensional reference map of human T cell activation in health for analyzing T cells in disease. For the entire paper, click here.